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The strain was maintained as spore suspension in 5% corn meal medium at 4°C.
The streptomycetes were maintained as spore suspensions in 30% (w/v) glycerol at −20°C [ 49].
Streptomyces isolates were purified and maintained as spore suspensions in 20% (v/v) glycerol at −20°C for subsequent investigation.
Stock cultures of C. acetobutylicum were maintained as spore suspensions in sterile 15% (v/ v) glycerol at −20 °C.
Bacterial cells were maintained as spore suspension on 60% (v/v) sterile glycerol at the ratio 2: 1 (culture: glycerol) and stored at -40°C prior to use.
Similar(55)
Streptomyces spp. strains were maintained as spores in 10% (v/v) glycerol and propagated in MYM and SFM agar media [ 14].
Stock cultures were maintained as spores suspended in 20% (v/v) glycerol, 0.8% (w/v) NaCl with 0.025% (v/v) Tween 20 at −80°C.
Cells were maintained as spores on solidified 2×YTG (16 g/l Bacto tryptone, 10 g/l Bacto yeast extract, 5 g/l glucose, 5 g/l NaCl, and 12 g/l agar) plates.
The strain is maintained as frozen spore suspensions at -80°C in 20 % glycerol.
Clostridium acetobutylicum ATCC 824 was maintained as a spore suspension in 6% (w/v) corn mash at 4°C.
The cultures were maintained as a suspension of spores and hyphal fragments in 15% (v/v) sterile glycerol at −20°C for long-term preservation.
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