Sentence examples for maintained as monolayers from inspiring English sources

We found no significant difference in the secretory responses of αTC1 cells maintained as monolayers or as cell clusters.

The cells were maintained as monolayers in plastic flasks in DMEM supplemented with 10% fetal bovine serum, 15 mmol L−1 l-glutamine, 100 units mL−1 penicillin, and 100 g mL−1 streptomycin and grown in a humidified incubator with 5% CO2 at 37 °C.

The cells were maintained as monolayers in Dulbecco's Modified Eagle's Medium with 4 mM L-Glutamine, 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, supplemented with 10% fetal bovine serum and 5% penicillin-streptomycin solution at 37°C and 5% CO2, and harvested by using 0.25% (w/v) Trypsin-EDTA solution.

These cells were individually maintained as monolayers at 37°C in 95% air, 5% CO2.

Cells were maintained as monolayers in an incubator within humidified atmosphere of 95% air and 5% CO2 at 37°C.

LMM3 cells were maintained as monolayers at 37°C in 5% CO2 in MEM supplemented with 5% FCS.

The cultures of the PC12 cells were maintained as monolayer in RPMI 1640 supplemented with 10% (v/v) heat inactivated (Gibco) horse serum, 5% (v/v) fetal bovine serum and 1% (v/v) penicillin/streptomycin (Thermo Technologies, New York, NY,USA) and incubated at 37 °C in a humidified atmosphere with 5% CO2.

Mouse pituitary GHFT1 cells [45] for FLIM experiments (Table 2, Figure S2) were maintained as monolayer cultures in DMEM containing 10% newborn calf serum.

BHK21, HEK293, BEAS-2BS, HeLaS3, Huh7, Hepa1-6, A549A549 cells were maintained as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal calf serum, 100 µg/mL penicillin, and 100 U/mL streptomycin, as recommended by the manufacturer (GIBCO, Gaithersburg, MD).

Cells were maintained as monolayer cultures at 37°C in an atmosphere with 5% CO2.

The HT-29, MT-1, Kelly, HUVEC and MRC-5 cells were maintained as monolayer cultures.