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These are maintained as high-copy linear mini-chromosomes.
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The second plasmid is maintained at high copy number as an episome and achieves high levels of expression of the target gene [ 18, 19].
In addition, there is a high copy origin of replication from pBluescript to allow easy manipulation of the vectors as high-copy plasmids before subcloning the BAC inserts which are then maintained at single copy.
This distribution has been used to separate isolates of M. tuberculosis into two groups: isolates with six or fewer copies of IS6110 are classified as low-copy isolates and those with more than six copies as high-copy isolates.
BACs are vectors derived from the F factor of E. coli that are maintained as low copy replicons.
Within land plants, gene copy number expansion continues within select groups, while several groups are strictly maintained as one gene copy per genome.
Originally, MITEs were described as high copy number, short elements in plants [ 39], reaching thousands of copies.
This plasmid, pESC-Leu2d::yNhANT4, is maintained both at high copy and expresses the gene under control of the strong Gal1 promoter.
We anticipated that the C. bescii shuttle vector would likely replicate in C. hydrothermalis and might be stably maintained at a high copy number.
Table S3 provides statistics (by counts and by kinds) of the high-copy (counts ≥3) as well as low-copy (counts <3) sequ-seqs.
Median lifespan of the SJL strain high-copy SOD1G93A mouse is 130 days, as originally characterized by Gurney [12], and as maintained by the Jackson Laboratories (personal communication).
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