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Mated females were generated and maintained as for lifespans.
Thereafter cells were maintained as for SP analyses except, before fixation, they were treated with their respective media supplemented with 2 µM cytochalasin D (Sigma-Aldrich Co., St . Louis Mo) for 14 hours to induce binucleated cell formation.
The PABA concentration was maintained as for the first study.
For age-dependent locomotion, mutants were synchronised and maintained as for lifespan experiments.
Flies were collected and maintained as for lifespan assays but without recording deaths.
Control experiments were also maintained as for the mixture of 25 μl NTE buffer and 5 μl viral suspensions.
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They are nicely maintained as well, ideal for marinating in your pretty mouth.
This window has been maintained as far as possible for all the examinations of the same patient.
The cell lines derived from ascites cells were maintained as above for the TOV derived cell lines.
This rule is maintained as much for your protection as for the Company's.
For proper emulsification, the emulsion was maintained as such for 45 min at stirring rate of 800 rpm and 60 ± 2°C.
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