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Where present and of high quality, postacquisition support facilitates regular maintenance and repair; where absent, it reduces stove functionality, with IS falling into disuse and not being maintained, repaired, or replaced as appropriate.
All strains were maintained as frozen stocks and then cultured on the appropriate media.
All strains were maintained as frozen glycerol stocks, and were sub-cultured into Luria Bertani medium with appropriate antibiotics (50 µg mL−1 kanamycin, 100 µg mL−1 ampicillin).
These are now maintained as historic monuments.
Age-matched rats were maintained as controls.
Embryos were maintained as standard method.
Cells were maintained as recommended by suppliers.
S2 cells were maintained as described [17].
ESCs were maintained as described [1].
Neuron cultures were maintained as described above.
Cells were maintained as described [5].
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