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KG-1a were maintained as above but with 20% FCS.
For rescue experiments, Ack-RNAi-PC12 cells were grown and maintained as above described.
The KG-1a cell line was maintained as above with 20% FCS.
Non-adherent cells were removed, and adherent cells were maintained as above and expanded in up to 6 passages.
The pig and human cell lines were maintained as above.
Cells were seeded in 6-well plates and maintained as above in culture medium without antibiotics.
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During the whole procedure blood glucose levels of 380 450 mg/dl were maintained as well as PaO2 levels above 200 mmHg.
Fish maintained as described above were anaesthetised (Benzocaine, 10 mg L−1) and bled from the caudal vein to obtain serum for analysis.
Following the final radiation, the CaP cell lines were maintained as the above culture method in a humidified incubator at 37 °C and 5% CO2 for ∼35 days for recovery.
Neurospheres were maintained as described above (see Neural stem cell cultures) and treated with GDF11 (50 ng/ml) or vehicle (4 mM HCl, 0.1% BSA, 0.5X DPBS) for 7 days.
General anaesthesia was induced and maintained as described above.
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