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In mitogens and serum, the NS/PCs became adherent, continued to proliferate, and either maintained an undifferentiated phenotype or differentiated, mainly as astrocytes (Fig. 3A).
A significant proportion of donor cells maintained an undifferentiated phenotype, while the rest differentiated into neuronal and glia cells (Fig. 2D and E, Supplementary Material, Fig. S3).
The phenotype of the cells transplanted by intravenous injection was similar to the intrathecal administration; the engrafted cells maintained an undifferentiated phenotype or differentiated into neuronal and glia cells (Fig. 3D and E, Supplementary Material, Fig. S6).
Enhancer of Zeste, Drosophila Homolog 2 maintained an undifferentiated stemness phenotype in ET (Richter et al, 2009), implicating that EZH2 might have a central role in ET pathology (Burdach et al, 2009).
They maintained an undifferentiated state expressing endogenous Oct-3/4, Sox2, Klf4, c-Myc, Nanog, and Lin28, and showed a normal ability to differentiate into three germ cell layers both in vitro and in vivo that was equivalent to that of marmoset ES cells (Sasaki et al. 2005).
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Former music video director McG maintains an undifferentiated frenetic and relentless pace with sub-Matrix action sequences that now look very shopworn.
These dental pulp-derived cell lines are stable and maintain an undifferentiated phenotype under long-term standard culture conditions.
Four hPSC lines propagated in the microfibrous scaffold for 10 consecutive passages were capable of maintaining an undifferentiated phenotype as demonstrated by the expression of stem cell markers and stable karyotype in vitro and the ability to form derivatives of the three germ layers both in vitro and in vivo.
This suggests that Ring1b has an important role in maintaining an undifferentiated state of ES cells.
These cells produce differentiating cells and also maintain an undifferentiated state by undergoing self-renewal division.
These data suggested that bFGF-hFLSCs produced TGF-β1 factors required by hES cells to maintain an undifferentiated state.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com