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The cultures containing cells with mast cell morphology remained as such despite maintained Lhx2 expression.
Moreover, the HPC lines derived from bone marrow that engrafted stem cell-deficient mice maintained Lhx2 expression in vivo and caused myeloproliferation [ 21].
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In contrast, young HSC cultures maintained CD34 expression (Fig. 4A), and all young CD34+ cells expressed β-catenin (Fig. 4B).
However, a few β-catenin protein-null cells still maintained Lmx1a expression (Fig. 6f',f″) in E12.5 β-cateninlof embryos.
The maintained Hes1 expression during differentiation of thyrocytes and C-cells as well as in the mature tissue differs from decreasing Hes1 expression in endocrine pancreas development [19].
GFP+/DsRed+ fused cells maintained CD31 expression, (D)), meaning that the endothelial phenotype was dominant.
MG132 and ALLN, which inhibit proteasome activity, effectively maintained TSC2 expression in the presence of TR3 (Fig 5C, top).
This stands in contrast with maintained COX4 expression levels in PC12-ND6 cells after 48 h of serum deprivation.
Similar to control SCs, LRCs from TetO-H2B-GFP mdx TetO-H2B-GFP mdxd Pax7 expression in viTetO-H2B-GFP mdxLRCs turned on muscleFig. 5E; supplemaintainedterial Fig. S4).
These data demonstrate that maintained CD28 expression on CD4+ T cells is required for full effector CD4+ T cell populations and clearance of C. rodentium.
Similar to cells grown in T cell medium, over 80% of MuSCs expanded in cytokine medium maintained Pax7 expression up to passage 20.
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