Field experiments showed a dramatic decline in the fluorescence yield with rising light intensity in both drying and artificially maintained wet plots.
Crusts were formed following incubation (25°C, 30 µmole photons m−2 s−1) in Petri dishes on filter paper maintained wet by periodic additions of water, and continued to grow in thickness for up 48 h (about 0.1 0.2 mm thickness in the wet state).
To overcome our inability to measure CO2 or O2 exchange in the natural crusts, we used artificial crusts where Microcoleus suspensions were soaked onto nitrocellulose filters (see [15]) that were maintained wet during exposure to 500 µmol photons m−2s−1 of blue and orange light.
The articular surface was maintained wet with PBS during the embedding process.
After light treatment, the thalli were maintained wet in darkness (to allow V-cycle re-epoxidation) for another 60 min. Samples were incubated in the dark for 24 h in glass hermetic chambers, containing a NaCl saturated solution (75% Relative Humidity -RH-).
For microscopic observations, the fibre scaffolds are either transferred to glass slides, maintained wet and observed with standard non-immersion objectives (without the addition of a cover slip); or for imaging at longer working distances, they are submersed in fresh growth medium, followed by imaging using a water-dipping objective.
Growth of the bog does not necessarily stop at this stage for, if rainfall is sufficient, the water-retaining properties of Sphagnum are sufficient to maintain a wet, stagnant environment above the original water table represented by the lake surface.
Environmental SEM (ESEM) is one special type of SEM that can maintain a wet environment within the sample chamber, which provides a novel platform for single cell analysis.
Management of the canal site to maintain a wet anoxic sediment, rather than abandonment and dereliction would provide a more sensitive and less costly solution.
Shellac is usually applied with a sponge, to maintain the wet edge.
Be sure to maintain a wet edge as you apply each band, working quickly across each face of the wood, one band at a time.
