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Next, the raw transcriptome was filtered to discard poorly supported transcripts, and to maintain transcripts with strong evidence of protein coding regions and reasonable support for expression.
Next, the raw transcriptome was filtered to discard poorly supported transcripts and maintain transcripts with strong evidence for protein-coding regions and reasonable support for being expressed.
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Survival requires maintaining transcript levels of essential genes above a threshold value.
This implicates that alternative splicing of some genes involved in maintaining transcript and protein stability is regulated by early fruit development.
The Ovation RNA-Seq V2 kit is able to amplify small quantities of RNA from tissue samples while maintaining transcript levels nearly identical to those from matched unamplified input RNA, demonstrating an overlap of 93% when comparing each matched pair individually.
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The general conclusion that could be drawn from these articles is that either IVT-based or Nugen-like RNA amplifications were globally able to maintain relative transcripts abundance with only slight differences.
These results suggest that MUC16 is required to maintain normal transcript levels of Muc1.
In this work, we show that MES-4 and DRM oppositely regulate a common set of genes to maintain proper transcript dosages for germ cells.
However, our finding that these were disproportionately NOVA-mediated actions to maintain neuronal transcript levels (215 vs 14) is consistent with an overall action linking alternative splicing suppression of NMD exons to steady-state transcript levels.
These authors found that, at least in these two species, duplicate copies of genes are more likely to be retained if the expression level is high than if it is low, presumably in order to maintain high transcript levels.
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