We then addressed the question of how these CGIs maintain their CpG rich sequences because methylated CpG dinucleotides convert to TpG dinucleotides more frequently than hypomethylated CpG dinucleotides.
As a result, we were confronted with the question of how CGIs with high methylation (HM) in somatic cells (or germline cells) can maintain their CpG rich sequences because methylated CGIs have more a rapid CpG → TpG/CpA substitution.
Therefore, we wondered how methylated CGIs in germline or non-germline cells maintain their CpG-rich sequences.
These results suggest that approximately 60 80% of CGIs with H1-HM in all the genomic positons maintain their CpG-rich sequences by a low CpG → TpG/CpA substitution rate.
These results also suggest that more than half the numbers of CGIs with H1-HM in all the genomic positions maintain their CpG-rich sequences by a low CpG → TpG/CpA substitution rate caused not only by their SPM-LM but also by another mechanism, such as CpG selection.
More than half the numbers of CGIs with H1-HM in all the genomic positions maintain their CpG-rich sequences by a low CpG → TpG/CpA substitution rate caused mainly by their SPM-LM, but when these have SPM-HM, CpG selection and relatively higher TpG/CpA → CpG fixation partly compensate them for frequent CpG loss.
These results suggest that approximately 60 80% of CGIs with H1-HM in all the genomic positons maintain their CpG-rich sequences by a low CpG → TpG/CpA substitution rate caused not only by their SPM-LM but also by another mechanism, such as CpG selection (Additional file 1: Table S1).
Considering these results, CGIs with SPM-HM may maintain their CpG-rich sequences, at least in part, by CpG selection and a slightly high TpG/CpA → CpG, fixation or they might disappear from the human genome in the future, because they have extremely frequent CpG → TpG/CpA fixation.
So when the rich maintain their money and investments, their increased net worth redounds to wages and job opportunities.
It means the filthy rich can maintain their lifestyles while buying virtue at a cost few of them will notice.
