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The parasite was passaged in BALB/c mice before the experiments to maintain the virulence.
The question of whether to maintain the virulence of tubercle bacilli through animal passage may have its roots in the personal, legal, and scientific disputes between Robert Koch and his student Emil von Behring in the late 19th and early 20th century [4], [5], [6].
For example, deletion or silencing of a class of enzymes that are implicated in virulence is not hindered by unknown genes of the same class which maintain the virulence effect.
To maintain the virulence, the fungus was inoculated to Arabidopsis seedlings and re-isolated from the infected tissues periodically [ 77]. A. brassicae sporulates heavily in Potato Dextrose Broth (PDB; pH 6.5-6.7).
This deletion also diminishes the bacteria's ability to grow inside the lungs in murine infection models, suggesting that this pump is required to maintain the virulence of the tubercle bacilli [ 17].
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However, to maintain the amoebic virulence, trophozoites were instilled in mice; seven days later, the brains were recovered in Bacto Casitone medium with antibiotics.
The study of serogroup O174 isolates also demonstrated that the virulence gene content of these evolutionarily divergent strains is similar, confirming the idea that multiple independent lineages of STEC have acquired and maintained the same virulence gene repertoire (36 ).
This indicates that compensation by the rhl QS system could override this hierarchy and maintain the expression of QS-dependent virulence factors in spite of a non-functional central las system.
These features allow the mutants to maintain the peroxidase activity required for virulence, and to resist killing by isoniazid.
In addition, in vitro experiments, such as one-step growth curves, and in vivo virulence studies in mice showed that recombinant Luc+viruses maintained the phenotypic and virulence properties of the parental viruses.
Also it maintains the levels of virulence and confers resistance to INH simultaneously [ 17].
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