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Aliquots were counted for total and viable cell numbers on the specified days and fresh media was added to replenish and maintain the total volume of media in the flask.
When the PCR result was unsatisfactory due to low DNA concentration, up to 0.5 μl DNA template was used; when DNA template volume was increased, dimethyl sulfoxide volume was reduced to maintain the total volume of 10 μl, accepting a slight shift in the overall ratio of ingredients.
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The effect of change in the geometric distribution of the TCEs on the performance of the heat sink was studied while maintaining the total volume of TCEs constant.
To assess the effect of concentration of extract on the formation of AgNPs, the volume was varied from 1 to 15 mL by maintaining the total volume of the reaction mixture as constant (20 mL).
For the tandem reactions of hydrolysis followed by decarboxylation, 2 μM OleTJE and 1 mM H2O2 were supplemented into the hydrolysis reaction while maintaining the total volume of 100 μl.
Let T be the age at which planted trees within a large area start to spend all of their energy on maintaining the total mass, so that volume growth after T is effectively zero within the area.
When reactions excluded a specific component, nuclease-free dH2O was substituted to maintain the total reaction volume.
This paper investigates the tensile behavior of the recently developed ambient-cured one-part engineered geopolymer composites (EGC) incorporating different hybrid combinations of steel (ST) and polyethylene (PE) fibers while maintaining the total fiber volume at 2%.
The ultrafiltration flow rate and the replacement fluid flow rate were both 0.15 ml/minute, maintaining the total blood volume consistently stable at 15 ml.
After that, the peptide solution was diluted with HEPES to its final peptide concentration in the plate well, and the NaOH content was maintained at <10% of the total volume.
Then the solution was replaced with fresh buffer solutions and the total volume maintains the same.
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