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To maintain the temperature of the preparation during ischemia, hearts were immersed in warm Tyrode's buffer (37°C).
Although bonding resin to enamel and dentin protected by a barrier impermeable to acids may maintain the retention for restorations, preparation of impermeable hybridized dentin is not as simple as for enamel as dentin contains more organic substances and water which can interfere with the diffusion of monomers [ 17, 20, 21].
Following each 2-min filtration with Microcon YM-30, each filtrate was recombined with the original granular preparation to maintain the initial volume.
Similar aging condition and calcination temperature were maintained for the preparation of Ce TiO2 sample.
The fluorescence spectroscopy analysis of the fluorescent nanocapsules and fluorescent lipid-core nanocapsules showed that the fluorescence property is maintained after the preparation of these formulations (Figure 6).
The biofilm is not visible due to SEM preparation techniques necessary to maintain the latex layer.
Concentration of the contaminant in the lower aquifer was about 197 ± 23 mg L−1 and it was maintained the same during preparation of anaerobic cultures.
An Immunization Team was created consisting of one physician and one study coordinator and was responsible for maintaining the blind during vaccine preparation and administration.
The removed cardiac tissue was kept in a buffer solution to maintain pH till the preparation of Western blotting analysis.
The lens cell membrane preparations maintained the native double membrane architecture of the junctional microdomains.
Moreover, care was taken to limit Fischer esterification conditions during sample preparation by maintaining the pH of digestion buffers >8, by avoiding exposure of samples to high concentrations of methanol in the presence of acetic acid, and by avoiding methanol as a co-solvent during LC separation of the peptides.
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