Kinetic modeling was then used to predict the conditions required to maintain the optimum CL levels and high cell concentrations in the reactor, based on the kinetic parameters of the WTC156T strain.
Because Cl-1 and Cl-2 interact with the backbone amides of ligands to the Mn4CaO5 cluster, at D1-E333 and D1-H332 for Cl-1 and at CP43-E354 for Cl-2, it has been proposed that Cl– functions to maintain the structure of the Mn4CaO5 cluster.
Cl- counter-ions were added to maintain the system charge-neutral.
If the scallop height is greater than the given value, more CL points are added to this region to maintain the required surface finish.
In order to maintain the pH gradient, tumor cells express ion transport proteins, including vacuolar H+-ATPase, Cl-/HCO3 - exchanger and Na+/H+ exchanger [ 8, 9].
Pipettes filled with high concentrations of Cl− for GABA-induced current recordings were used to maintain the Cl− equilibrium potential close to 0 mV, thereby facilitating the observation of GABAR-mediated whole-cell currents at resting potentials.
The antibiotic gramicidin was used in order to maintain the intracellular Cl− concentration [Cl−]i [ 24].
High Cl− uptake in the root (Fig. 5G) is a compensating mechanism that tries to sustain the root growth, as well as to maintain the charge balance.
Sphere-forming cells in group CL, however, maintained the expression of these undifferentiated markers.
The resulting CL-Pgp maintained the expression and full activity in yeast functional assays, and the purified protein displayed drug-stimulated ATPase activity indistinguishable from that of WT Pgp.
Also, the conversion into MAPbI3(Cl) is completed while maintaining the [110] orientation without remnant, as verified from the diffraction in Fig. 3a (magnification in Additional file 1: Figure S2(a)).
