They are supervised by a registered nurse, who dispenses fresh needles, swabs, sterile water to cook the drugs and advice on how to maintain veins.
Three types of swabs were sampled from each pig (nasal, tongue and rectal swabs) and maintained in Cary-Blair transport medium (Oxoid, UK) in ice box before being processed in laboratory.
Where other labs maintain huge server farms of coded genes, Preti has two fridges covered with cartoons and crammed with saliva, urine, swabs of exudates and other unmentionables.
Maintain, maintain, maintain.
Home swabs were maintained at room temperature until the nearest clinic visit and then stored as above.
At the end of incubation, nonmigrated cells were removed using a cotton swab; invaded cells maintained in the upper chamber were fixed with 4% formaldehyde and stained with 2% crystal violet.
Briefly, anaesthesia was maintained with isofluorane, knees were swabbed with 70% ethanol and a 5 μl volume (treatment) was injected into the synovial space.
Before the laboratory experiments, ashes were autoclave-sterilized. Fresh bacteria for spiking experiments were isolated by streaking swabs from glycerol stocks maintained in the CVD repository onto selective media and culturing plates overnight at 37°C.
Swabs from X. laevis maintained in U.K. research institutions were tested for infection by RT-PCR assay (n = 1795 swabs/27 laboratory populations).
The 10% lung homogenate (PU243) and nasal swab preparation (PU542) were applied onto MDCK cells maintained in Eagle's minimum essential medium containing 4 μg/mL trypsin and 0.3% bovine serum albumin (Sigma, St . Louis MO, USA).
