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Hydroxyurea treated cells failed to maintain supernumerary centrosomes and none of the cell lines analyzed became aneuploid despite the initial presence of a large percentage of cells with amplified centrosomes.
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Specific antibiotics were added to the medium when necessary to maintain the supernumerary chromosomes.
Finally Myb encodes a proto-oncogene with transcriptional activator activity involved in centrosome cycle which is required to sustain the appropriate rate of proliferation, to suppress formation of supernumerary centrosomes, and to maintain genomic integrity [ 42].
Just maintain.
Maintain, maintain, maintain.
Exogenous juvenile hormones (JH) application at this time maintains the larval secretory program, resulting in supernumerary (extra) instars.
On the contrary, ectopic expression of Aurora-A/STK15 caused supernumerary centrosomes that were maintained over time and likely generated subsequent aneuploidy.
Thus, paralysed patients could experience a supernumerary prosthesis as part of their own bodies while maintaining ownership of their real limbs.
Abnormalities in budding during the embryonic development may lead to the formation of supernumerary bronchi when continuity with the developing tracheobronchial airway is maintained, or may lead to the development of bronchogenic cysts when this connection is lost [ 1, 3].
24, 27, 72, 73, 75– 79, 81, 149 Restriction of Shh signalling appears to be a key event implicated in the rudiments' regression, 27, 75 and SHH and FGF4 production maintained in a diastema bud in Spry null mouse embryos enable it to develop into a supernumerary tooth.
These results suggest that supernumerary centrosomes can be induced in HCT-wt cells as well as in their derivatives, but they are not maintained over time.
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