To overcome the thick Gram-positive cell wall, mild or brief pretreatment using cell-wall-weakening agents, such as lysozyme, glycine, dl-threonine, or penicillin G, is commonly required to achieve optimal electrotransformation while maintaining sufficient cell viability [ 23, 24].
It is also known that the type IV cells continuously undergo replication, as shown by Ki67, Shh and BrdU staining, so as to maintain sufficient precursor cell numbers continuously available to receive neuronal signals 38, 39.
Although most women with PCOS maintain sufficient beta-cell function to prevent deterioration in glucose tolerance, a significant proportion, especially those with first degree relatives with type 2 diabetes, demonstrate abnormal beta-cell response in response to glucose challenge or meals.
FACS analysis of thymocytes revealed no significant differences in the frequency of any thymocyte subsets (Figure 4C), suggesting that expression of DKK1 in TECs does not lead to stage specific blocks in T cell development but rather a general loss of thymic capacity maintain sufficient numbers of T cells, possibly due to a loss of epithelial niches.
A plausible reason for the discrepancy in proliferative capacity may be that H1111R mutant lymphoblastic cells express very little functional Zfp335, while blt/ blt cells maintain sufficient Zfp335 activity such that they are capable of normal proliferation and growth.
Cells with reconstituted telomerase activity maintain sufficient telomere lengths that allows cells to not only circumvent replicative senescence, but escape from crisis and become truly immortal [ 13].
Cells can maintain sufficient PCs to bind with Pb.
To maintain sufficient oxygen diffusion to all cells inside the section, investigators now favor slices of no more than 400 μm thickness (3), to avoid potential exposure to hypoxic conditions.
Taken together, these results led to the conclusion that PUM2 is required to maintain sufficient amounts of Aurora-A in the cells, which is indispensable for the interaction between Aurora-A and PUM2.
However, since the cells fail to maintain sufficient hormone quantity to offset insulin resistance, hyperglycemia arises, which indicates failure in insulin secretion.
