Zebrafish were maintained, staged and injected according to standard procedures [16].
Zebrafish were raised and bred using standard protocols, including maintaining staged embryos at 28°C [41], [42].
When product market space matures and the market share between competitors becomes fixed, the company enters the maintain stage.
Running a medium-sized $50 million sales department in the compete stage requires a much different skill set than leading a large billion-dollar sales organization in the maintain stage.
42.9±13.7% of cells exposed to 18% stretch exhibited Ca2+ sparks (6 out of 14 experiments, Fig. 7A and D), compared to 6.3±6.3% of cells exposed to LY294002 30 µM(1 out of 16 experiments)in stretch maintaining stage (Fig. 7B, left and D), supporting a role of PI(3 K-dependent signaling in the signaling pathway leading to Ca2+ sparks.
We maintained stage X blastoderms, GSCs, and CEFs as reference samples.
According to this model, chronic fatigue symptoms develop through a developmental and cumulative process that involves different psychobiological factors contributing to triggering, predisposing, and maintaining stages.
Such cross-species emergence proceeds in various stages — after exposure (stage I, Figure 1), the pathogen must be able to infect the novel recipient host (stage II), this single infection must result in onward transmission to con-specifics (stage III), and such transmission must be maintained (stage IV).
Until recently, a consensus has been maintained on staging the 2012 Olympics.
The unique attributes of P. vivax biology, particularly the capacity of the dormant liver stage, the hypnozoite, to maintain blood-stage infections even in the absence of active transmission, make blood-stage vaccines particularly attractive for this species.
We maintain a staging environment that is very similar to the production one (including https support and services topology).
