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For decades, researchers have used embedding media such as hydrogels to maintain samples in place.
Lower porosity and impurity levels may make it difficult to produce and maintain samples with the smallest nanocrystalline grain sizes.
Marine sediments retrieved with pressure coring systems such as those used by the Integrated Ocean Drilling Program maintain samples at in situ hydrostatic pressure throughout recovery.
For all experiments, a stage-top incubator (Tokai-Hit, Japan) was used to maintain samples at 37C.
It is also a non-hardening medium that can maintain samples for up to several weeks at 4°C.
Blood was collected into EDTA-containing tubes and immediately placed in IsoRack cool packs (Brinkmann Instruments, Westbury, NY) designed to maintain samples at 0°C for > 6 hr.
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Capture also occurred in autumn (September to November) to maintain sample size from dropped collars or fatalities.
The third criteria helped maintain sample sizes because each lek is not counted every year but most are counted at least once every three years.
Blood samples were processed and stored per protocol to maintain sample integrity.
Typically, once a specimen is collected, samples are kept cold and then frozen to maintain sample integrity.
This procedure allowed us to maintain sampling information while simultaneously analyzing the groups in a comparable manner.
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