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Spontaneously breathing male Wistar rats (300 350 g body weight) were anesthetized by 5%% isoflurane in room air in an induction chamber and then placed on a heated mat to maintain rectal temperature at 37 °C throughout.
A heating blanket was used to maintain rectal temperature at 36.5°C to 37.5°C.
A heating pad was used to maintain rectal temperature at 37.5 38.5°C.
Reasons will be recorded, and might include clinical, EEG and imaging evidence of severe, irreversible brain injury, or continued inability to maintain rectal temperature in the desired range.
Rats were placed on a homeothermic blanket system to maintain rectal temperature between 36.8 and 37.8°C for the duration of the experiment.
The rats were anesthetized with intraperitoneal pentobarbital (50 mg/kg, Trapanal®, Nycomed, Germany) associated with local anesthesia for each incision (Lidocaïne®1% Astra Zenecca) and placed on a homeothermic blanket system to maintain rectal temperature between 36.8°C and 37.8°C.
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After surgery, animals were placed in cages above a heating blanket to maintain rectal temperatures above 36°C for 1 2 h with active monitoring.
The temperature of the animal was controlled by warm air where the heating of the air was controlled by a feedback system to maintain the rectal temperature at 37 °C (SA-instruments, Stony Brook, USA).
The animals were pre-warmed with a panel heater to maintain their rectal temperature at 37°C during the determination of cardiac parameters by echocardiography.
The incubator heater will be adjusted during transport to maintain a rectal temperature as close to 37.0°C as possible.
The incubator heater will be turned on and adjusted if necessary to maintain the rectal temperature between 33 and 34°C.
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