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Cell suspension is further homogenized using a homogenizing apparatus and buffers that maintain physiological conditions.
In a recent review the role of microRNAs and their integration into multilayered cellular networks functioning to maintain physiological conditions was discussed (Rottiers and Naar, 2012).
Such ex vivo bioreactors maintain physiological conditions to study product prototypes for better performance in preclinical and clinical studies by minimizing product development cycle; also this step aids in reducing preclinical costs by implementing different prototype modifications in ex vivo studies.
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HEPES is a Good buffer for maintaining physiological conditions in cell cultures but poses some problems like long-term stability especially when exposed to humid air and light.
The present work demonstrates the design and function of ex vivo bioreactor system capable of maintaining physiological conditions along with hemodynamic forces to mimic the in vivo vascular conditions.
VGSCs play a pivotal role in maintaining physiological conditions and higher cognitive functions, but also mediate hyperexcitation and neurodegeneration, and their dysfunction is implicated in a number of neurological diseases.
In the present study oxygen consumption and mechanical parameters of isometrically contracting rabbit papillary muscles (1 Hz stimulation frequency) were investigated at varying interventions while maintaining physiological conditions (37°C; 2.5 mM Ca2+) to study the effects of NO on energetics.
Bioreactors constitute and maintain physiological tissue conditions at desired levels, enhance mass transport rates and expose cultured cells to specific stimuli.
Control and pertussis toxin-treated colonies were imaged with a Carl Zeiss Axio Observer Z1 and AxioVision version 4.7 software (Carl Zeiss) and configured with a linearly scaled stage and an XL S incubation unit (Carl Zeiss) to maintain physiological atmospheric conditions at 37°C and 5% CO2 with humidification.
They are considered to have reached the extreme limits of human life span but still show relatively good health conditions to maintain physiological function and escape the common fatal diseases [ 7].
Specifically, loss of HSI2 function results in plants better able to maintain physiological water potential under limiting water conditions, as indicated by reduced wilting and higher leaf RWC, while overexpression of HSI2 yields a complementary phenotype.
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