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The benefits of the STZ-induced diabetic mouse model include that it allows one to induce diabetes in genetically altered mice, maintain mice in a controlled environment, regularly monitor and directly measure serum and bone factors, obtain bone samples for high-resolution analyses, and chose the time of diabetic induction (compared to waiting for the diabetes to occur in spontaneous models).
a. Maintain mice in gnotobiotic isolators.
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The decision not to use shorter conditioning sessions also eliminated the undesirable and potentially complicating factors that could have resulted from maintaining mice in continuous isolate housing outside of the conditioning environments.
These results indicate that Mbd3 may be essential for maintaining mouse ES cells in an undifferentiated state.
We bred and maintained the mice in an animal pathogen-free facility at The Ohio State University Medical Center.
An n = 6 was maintained for mice in each group, unless otherwise mentioned.
Cells were maintained in mice in ascites form by successive transplantation of 6×10 cells/mouse in a volume of 0.2 ml in PBS [ 18].
Based on these data, we delivered insulin doses of 0.05 U/day to maintain diabetic NOD mice in poor glycemic control, and 0.2 U/day to maintain diabetic NOD mice in good glycemic control.
129S6/SvEv and C57BL/6J WT mice maintained in our mouse colony were the controls in the behavioral experiments.
In contrast, PGC-1α levels are maintained in mice lacking MKP-1 [ 15].
The non-culturable IOE was maintained in mice.
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