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This initial event leads to pathological changes in the activity of ion channels that maintain membrane potential.
When the mitochondria maintain membrane potential, JC-1 (green fluorescence) forms the J-aggregates (red fluorescence).
Within young cells, mitochondria are impermeable to most ions and solutes, allowing them to maintain membrane potential and store Ca2+[ 4].
PHB1 and PHB2 form large megadalton complexes within the inner mitochondrial membrane where they are thought to regulate mitochondrial morphology and maintain membrane potential (Artal-Sanz and Tavernarakis 2009a, b; Osman et al. 2009; Van Aken et al. 2010).
Frequency vs current intensity curves were plotted by measuring the average rate of action potentials in current-clamp during 500-ms long depolarizing steps of increasing intensity; a small DC current was injected to maintain membrane potential at −65 mV in between depolarizations.
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This may also be related to membrane depolarisation events observed after NH4+ application in NH4+-tolerant plants or to the higher energetic burden reportedly required to maintain membrane potentials in NH4+-sensitive species [ 44].
The intracellular chloride channel (CLIC) plays an essential role in cellular function, pH, electrogenic balance and maintaining membrane potential in organelles.
GIRK1 acts to conduct potassium ions into the cell rather than out of the cell, and play a role in maintaining membrane potential.
When electron transport function is inhibited, the ATP synthase can function in reverse such that it uses ATP generated by glycolysis to pump protons across the inner mitochondrial membrane, maintaining membrane potential (Appleby et al., 1999).
H+-ATPase activity is indispensable for maintaining membrane potential (MG Palmgren and P Nissen [ 20], and intrinsically higher H+-ATPase activity was shown to correlate with salinity tolerance in barley [ 38].
Indeed, as shown in [ 31], an ATP balance of 1250 mmol/h/half udder (60 mol/d/udder) can be expected to be used for other functions not accounted for in the models, such as maintaining membrane potential and synthesizing nucleic acids.
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