Preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity.
This suggest that the steering region at high salt is more limited, albeit maintaining its specificity.
One predominant sequence (iM10) was enriched during selection and showed remarkable protease resistance, high solubility and thermal stability while maintaining its specificity.
Before using this method, its sensitivity needs to be improved while maintaining its specificity.
Before using this method as a screening instrument for selecting patients who might benefit from structured care, its sensitivity needs to be improved while maintaining its specificity.
The A314P mutant of NaManD maintained its specificity for d-mannonate, but with a reduced catalytic efficiency (3200 M 1 s–1 to 60 M–1 s–1).
In male-derived S2 cultured cells (Schneider 1972), which are amenable to manipulation by RNAi (Clemens et al. 2000), the MSL complex maintains its specificity for X-chromosomal sequences; in this case, approximately two X chromosomes per nucleus are targeted due to tetraploidy/aneuploidy of the cell line (Hamada et al. 2005; Williams et al. 2007; Zhang et al. 2010).
Notably, to accelerate the kinetics of the machinery while maintain its high specificity, we investigated the sequence of DNA templates systematically and established a set of guidelines for the design of the optimum DNA templates, which allowed for instant detection of the target NTP at fmol level in less than 1 min.
Second, unlike DNA arrays, in which no secondary and/or tertiary structure is required for a nuclear acid probe (immobilized on a solid surface) to maintain its function and specificity, most proteins spotted on the solid surface do no maintain their function over time.
In these regions our method predicts many more nucleosomes, agreeing with the calls of Lee et al., while maintaining its high specificity.
