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This shows that signaling between CdxA expressing cells is normally needed to maintain its expression.
Regulating mechanisms that maintain its expression could turn to be a potential tumor regulator due to tumor suppressor functions.
In this way Nodal signalling is both upstream of the AVE, as it induces AVE gene expression [20], and downstream of this tissue, as some AVE genes, such as Bmp2, are required to maintain its expression.
Furthermore, our choice to express the fusion protein from an inducible promoter on a plasmid rather than from a modified chromosomic copy was made in order to strictly control the amount of the protein present in the cell, to maintain its expression level close to the physiological one or to overexpress Hfq-MT when it is necessary in order to characterize the biological system.
Mcl-1 is a highly unstable protein, requiring active protein synthesis to maintain its expression levels.
A reporter based on an endogenous β-catenin target gene can in that case truthfully report increased activity of the pathway, but reduction is not necessarily detected as other pathways could maintain its expression.
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Therefore, it is helpful to have a marker at hand, which maintains its expression qualitatively even in poorly differentiated carcinomas.
Supplementary Information Table S2 shows that cytochrome c maintained its expression before 48 hours of heat treatment.
We first examined the expression of Foxp3 since we had found that NOD APC were defective at maintaining its expression in CD4+CD25+ cells in vitro (Table 2).
We also note that TLRs stated to form at fatal stage and maintained its expression level after birth, which agrees with previous reports [29] [30].
In addition, in CHF, both HDAC1 and HDAC2 showed strong staining in fibroblasts while HDAC2 maintained its expression in cardiomyocytes.
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