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SYBR green I dye simplifies the experimental design but introduces the need for specific controls to maintain high specificity.
We utilised highly specific hybridisation probes and primers to maintain high specificity for target genes and thereby reduce false positive outcomes.
To maintain high specificity and allow probe binding and hydrolysis of a probe with low melting temperature, a two-stage touchdown PCR was designed with a first round of amplification at high temperature and a subsequent round at low temperature to allow accumulation of fluorescence.
The use of a stringent definition of a positive result was implemented in this study in order to maintain high specificity [13].
It is therefore essential to maintain high specificity in this context.
However, if the Tm is increased to maintain high specificity, then the test sensitivity will in turn decrease.
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Maintaining high specificity and consistent sensitivity across diverse HIV strains is critical for these assays to accurately detect HIV infection and expedite delivery of patient results.
The specific aim of this study was to identify a BBN analogue that can be radiolabeled with 177Lu and maintains high specificity for GRPr positive prostate cancer tumors in vivo.
A cutoff value of 2.70 µg/ml identified a modest subset of ALS patients (sensitivity: 32%) while maintaining high specificity versus controls (specificity: 78% vs. all study controls, 100% vs. mimic disease controls).
These autocatalytic strategies have resulted in unmatched sensitivities while still maintaining high specificity.
De Mita et al. (2006, 2007) have hypothesized that such purifying selection might have maintained high specificity of recognition and removed mutations deleterious to the establishment of symbiosis.
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