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Current techniques that maintain hepatocellular function in vitro with different biomaterials and geometries exhibit a low cell density and functional capacity per unit volume.
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We hypothesize that cultures with lower concentrations of rLSECs (5000 or 10,000) did not maintain hepatocellular functions due to lower heterotypic cell-cell interactions.
In these patients liver involvement may be the major manifestation of CF. However, portal hypertension and gastrointestinal haemorrages in CF are usually associated with a maintained hepatocellular activity.
The two features combine to cause portal hypertension and reduced hepatocellular function.
As PDRICG is dependent from both hepatocellular function and effective sinusoidal perfusion, this consecutively leads to reduced PDRICG.
In this study, we demonstrated that PDRICG is not correlated with classic liver laboratory tests; hence, it may provide additional information on hepatic blood flow and hepatocellular function.
Hepatocyte iNOS expression is a novel and, as yet, poorly described antioxidant mechanism that is cytokine and redox sensitive and that plays a pleuripotent regulatory role in hepatocellular function in the face of sepsis and shock.
Two possible explanations for this observation might be that either PDRICG does not reflect liver function at all or PDRICG gives additional information on hepatocellular function and hepatosplanchnic perfusion that is not 'detected' by the classic liver function tests.
The application of the FBA model to enhance hepatocellular function via.
The impairment of hepatocellular function observed in early sepsis appears to be due to upregulation of proinflammatory cytokines such as TNF-α [4], [5].
Thus, we can correlate increased amino acid uptake rates and changes in glucose metabolism to hepatocellular function at the stable stage of culture.
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