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Under these conditions the bacteria grow slowly, have less competition, and therefore may be under less selective pressure to maintain gene order.
An alternative possibility to that proposed by Liu is that lifestyle differences of the host-specific serovars are either inducing the rearrangements by increasing recombination frequency, or decreasing selective pressure to maintain gene order.
These observations suggest that the functional constraints that maintain gene order in metazoans would be actually very relaxed in O. dioica (Chavali et al. 2011), alternatively they may be indicative that this type of constraints do not exist at all in any metazoan.
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Despite high levels of divergence at the sequence level, these three species exhibit a striking conservation of gene order, suggesting that selection has maintained gene order among the trypanosomatids over hundreds of millions of years of evolution.
Our findings are supported by recent reports that suggest that XIST is expressed in adult well-differentiated cells in order to maintain gene repression [ 35- 39].
It is further proposed that purifying selection acts to maintain the gene order in Pelagibacter and that this is somehow related to genome streamlining.
The comparative analysis of orthologous gene-rich regions between both species revealed insertion of transposons exclusively in the pepper sequences, maintaining the gene order and content.
It is therefore critical that conservation programs acknowledge the role of coevolutionary processes between host and pathogens in determining infection outcomes and focus on maintaining gene flow among host populations in order to minimize genetic drift and the loss of resistance alleles (Altizer et al. 2003).
Eplasma, Gplasma and Fer1 maintain the Nuo gene order found in a number of other archaea including, Halobacterium sp., Sulfolobus solfataricus, and T. acidophilum[ 68].
Maintaining their original gene order, these genes were found on the opposite strand in Nymphon gracile compared to other arthropods.
To investigate whether the high number of candidate orthologs with Pgt maintained the same gene order, the Pt BAC sequences were aligned to the available Pgt contig sequences.
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