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A variety of amino-terminal fusions maintain enzymatic activity (or produce enzymatically active breakdown products) including those fused to codon 12 of NPT [ 48- 52].
In vitro reconstituted forms of topoisomerase with only the NH2-terminal domain deleted (topo70) or both the NH2-terminal and the linker domains deleted (topo58/6.3), maintain enzymatic activity [13].
Although RBD mutants of p110α fail to bind KRAS, they still maintain enzymatic activity.
By extracting total embryo protein under conditions that maintain enzymatic activity, we were able to perform protease assays using fluorogenic gelatin and collagen IV substrates.
Only over the past decade was convincing evidence found for these enzymes as to their ability to migrate to the nucleus where they maintain enzymatic activity 95, 98, 111, 113, 114, 116, 120, 129.
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GerP proteins encoded within this operon are not predicted to maintain enzymatic activities, and yet gerP mutants have severe germination defects in other Bacillus species [23].
The idea behind these efforts is to promote formation of lobular structures with apical-basal polarization (including bile canaliculi formation) of hepatocytes and additionally to maintain enzymatic activities.
This study has shown that three ligands that are closely associated with the T1 copper in SLAC play a key role in maintaining enzymatic activity.
This result is consistent with amino acid substitution analysis of BY-kinases and supports the notion that these enzymes evolve fast and only conserve at essential sites to maintain core enzymatic activity.
The results indicate that the pMAL ™ protein expression system enables the production of the exochitinase and endochitinase in such a way that they maintain their enzymatic activity even when bound to MBP.
3b Therefore, appropriate synthesis of nuclear NAD+ is required for the cells to maintain their enzymatic activity.
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