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An 8 g sample of WT, GT, or BT loose tea leaves were added to 100 mL boiling, deionised water and maintained at boiling for a period of 10 minutes.
Bring to the boil and continue boiling for five minutes.
Continue boiling for 60 minutes.
Avoid boiling for too long.
The mixture was then boiled for 4 h while periodically adding distilled water to maintain a volume of 560 mL.
Return to a boil and boil for 4 minutes.
Let it boil for six minutes.
Cover and boil for 3 minutes.
It was boiled for 5 min.
Then it was boiled for one hour.
Return to a boil, then boil for 1 minute.
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CEO of Professional Science Editing for Scientists @ prosciediting.com