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In addition, these two methods require much less space to incubate and maintain bacteria than conventional plate media.
HS-AFM imaging was conducted in a culture medium to maintain bacteria alive.
It was shown recently that, at least upon direct infection in vitro with Brucella abortus, human fibroblast-like synoviocytes are able to maintain bacteria replication and increase production of joint-damaging metalloproteases (Scian et al., 2011).
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Burnet prepared or maintained bacteria cultures for other researchers in the morning and was free to do his own experiments in the afternoon.
Wide dissemination of vertically maintained bacteria is common in arthropods [23], [24].
Another set of bacteria was treated with 0.02% SDS to maintain intact bacteria cell morphology.
Fernández-Marín et al. demonstrated that Atta and Sericomyrmex workers lacking visible bacteria bio-films responded to pathogen challenges by increasing metapleural gland grooming rates, whereas Acromyrmex and Trachymyrmex (which maintain abundant bacteria bio-films) displayed lower metapleural glands grooming rates.
This effect could maintain infecting bacteria in a susceptible state so they can be eradicated by other host defenses.
Antibiotic resistance marker genes are commonly used to select and maintain recombinant bacteria in the presence of antibiotics.
In summary, this study provides an example of how essential genes can be used in combination with non-antibiotic inhibitors to select and maintain recombinant bacteria.
The role of P66 in efficient dissemination is biologically significant in light of the natural transmission cycle required to maintain the bacteria in wildlife reservoirs, and is relevant to the development of disseminated disease in accidental hosts such as humans.
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