The viral genome is maintained autonomously in proliferating cells and each viral episome is replicated once per cell cycle during S phase [1], [2].
In DT40 cells, the tet-O HAC was maintained autonomously without integration into chromosomes (Fig. 2C).
These data show that STAR elements have spread through and been maintained autonomously within each staphylococcal genome.
FISH analysis showed that the HAC was maintained autonomously without any detectable integration into the host genome in three out of five randomly selected clones.
FISH analysis showed that the HAC was maintained autonomously in six out of six randomly selected clones (Fig. 4B and Supplementary Table S1).
The data show that STAR elements are associated with distinct flanking genes in each staphylococcal species, suggesting that they are maintained autonomously within each species, and that their positions within each genome are stable over time.
These results suggest that, when elicited in three dimensions, the NMps can organize themselves into structures that resemble the initial steps of spinal cord development and can be maintained autonomously for a number of days.
Several lines of investigation now support a model whereby the epidermis is regionally compartmentalised into functional units that are maintained autonomously (Ghazizadeh and Taichman, 2001; Levy et al., 2005; Page et al., 2013).
Although pDCW89 DNA methylated in vitro with M.CbeI transformed C. hydrothermalis JWCH006 to prototrophy, we found that the wild-type plasmid-encoded pyrF allele repaired the mutant pyrF locus in almost every case (Additional file 1: Figure S1), and the plasmid was not maintained autonomously over time.
In nature, biological systems have self-organization capability, i.e., homeostasis, as they autonomously maintain a relatively stable equilibrium state for operation of vital functions.
The proposed approach employs adaptive group testing techniques to autonomously maintain FPGA resource viability information as an organic means of transient and permanent fault resolution.
