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Each method has its own set of unique advantages and drawbacks, all with an inherent challenge to maintain assay sensitivity in the presence of potentially cross-reacting and competing amplification or target detection.
To maintain assay precision, samples with a CV > 12% were excluded.
Several quality control measures were implemented to maintain assay accuracy (2, 6).
Enzyme activity was assayed in an anaerobic chamber (COY labs, Grass Lake, MI, USA) using an Agilent 8453 spectrophotometer with Peltier temperature control module (part number 89090A) to maintain assay temperature.
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The results presented in this study demonstrate a successful automation paradigm resulting in an eightfold increase in throughput while maintaining assay performance characteristics comparable to the original assay.
As image-based analysis was preferred to improve data quality by eliminating potential interferences caused by fluorescent or quenching compounds, the original DAPI assay was further modified and optimized to significantly improve throughput, while maintaining assay precision and the magnitude and linearity of the fluorescent signal.
An analysis conducted as part of the recent Organisation for Economic Co-operation and Devaluation (ofCD) evaluathen of the immature rat uterotrophic assay indicated that isoflavone levels greater than 325 350 mg GEN equivalents/kg diet should be avoided to maintain optimal assay sensitivity and dynamic range (Owens et al. 2003).
Given these variations and factoring in the relatively narrow range of boundary iron concentrations used in this profile (the latter largely being determined by the need to maintain adequate assay signal), an overreliance on this filtering step to exclude series from further consideration carries the risk of losing viable scaffolds.
Since the method by which an mRNA species is measured will have a profound effect on the success of such a validation study, it is important to characterize and maintain the assay's performance, particularly its reproducibility and quantitative precision.
We wrapped the pipettes in aluminum foil multiple times (>3), to maintain the assays in complete darkness.
The immobilization of ConA to the solid-phase presumably maintains the assay stability by either stabilizing ConA and/or preventing unfolded ConA from aggregating with itself.
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CEO of Professional Science Editing for Scientists @ prosciediting.com