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It also is difficult to maintain and study appropriately some invertebrates under laboratory conditions.
The development of reliable cell culture methods to harvest, maintain, and study the cells of the cardiovascular system now allows for the investigation of dynamic structure and function relationships at the cellular and molecular levels.
Although non-human primates are physiologically representative model organisms to understand human parturition and preterm birth, the expense and time necessary to maintain and study these animals as well as their long gestation periods (greater than 5 months), the unfeasibility of genetic manipulation, and the lack or limitation of optimized reagents make their use less practical (Table 3).
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Animals used in this study were maintained and studied under protocols approved by the University of North Carolina Institutional Animal Care and Use Committee.
These mice were maintained and studied under pathogen free conditions in accordance with guidelines from the American Association for the Accreditation of Laboratory Animal Care and the Institutional Animal Care and Use Committee of Wyeth Research.
Many more Rubus molecular markers and other genomic tools are needed to map important traits, facilitate cultivar development, maintain cultivar identity, and study basic genetic and genomic mechanisms.
It has also been observed that primary human breast tumor cells can be maintained and effectively studied ex vivo if the cells are maintained in the presence of either a reconstituted BM or purified collagen I matrix [ 33, 34].
Three hundred and twenty-six patients undergoing potentially curative resection for CRC were identified from a prospectively maintained database and studied.
Blinding was maintained for patients and study centre and local sponsor personnel throughout the double-blind treatment period.
HIPPA compliance was maintained, and the study adhered to the Declaration of Helsinki.
They were housed in cage; a 12 h dark/light cycle was maintained throughout the study at room temperature (21 24°C), and relative humidity of 50 to 70% was maintained and acclimatized to study area conditions prior to 5 days.
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