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This was first derived from the study by Rivers and colleagues, in which most EGDT-treated patients had received RBC transfusion to maintain a hematocrit level above 30% [5].
In addition, the widely circulated recommendations of the Surviving Sepsis Campaign regarding blood transfusion in patients with septic shock include a recommendation for transfusion to maintain a hematocrit of more than 30%% in the presence of hypoperfusion in the first 6 h [41].
During and after CPB, additional RBCs were administered as needed in order to maintain a hematocrit value within our standard range.
Packed red blood cells (200 to 500 mL) were transfused as necessary to maintain a hematocrit level above 30% during CPB [ 26].
In addition, the widely circulated recommendations of the Surviving Sepsis Campaign regarding blood transfusion in patients with septic shock include a recommendation for transfusion to maintain a hematocrit of more than 30%% in the presence of hypoperfusion in the first 6 h [ 41].
After each patient was separated from CPB, RBCs were transfused to maintain a hematocrit level above 24% in cases of abnormal bleeding or to increase oxygen delivery in cases of persistent lactic acidosis after optimization of cardiac output with inotropes, vasoactive agents or both.
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Controls maintained a hematocrit of around 41% throughout the study.
Response was durable; among responding patients, the probability of maintaining a hematocrit < 45% without phlebotomy for 48 weeks and 144 weeks, respectively, was 85% and 61% (Fig. 1c).
Clinical symptoms are effectively relieved by phlebotomy, but the increased risk of cardiovascular morbidity is not ameliorated by maintaining a normal hematocrit [30].
In contrast, mutant mice maintained a low hematocrit for at least two more days and 25% of them died at day 4 or 5.
Fresh human red blood cells were obtained from Dutch National blood bank (Sanquin Nijmegen, NL; permission granted from donors for the use of blood products for malaria research), washed in serum free medium, and these were added to these cultures at parasitemias between 2 7%, thereby reducing the parasiteamia to 0.5% while maintaining a 5% hematocrit.
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