This will clear away any germs and maintain a hygienic environment for washing dishes.
To prevent any evaporation, the biosensor device was maintained within a petri dish with a cover, and extra cell medium within the dish.
Cells in coverslips were maintained inside a Petri dish with medium without glucose in an atmosphere of N2 95%/CO2 5% for 24 h (pilot studies demonstrated at this time cells showed morphological evidence of stress, cytoplasmic shrinkage and propidium iodide uptake (PI) demonstrating DNA damage and apoptosis.
CCL11 also impaired their performance on the fear conditioning and memory tasks, and inhibited the differentiation of neural stem cells maintained in a culture dish.
On the day of the experiment, a bundle was transferred to a dish containing skinning solution, maintained at 10°C, and single muscle fibres were dissected with the help of a stereomicroscope (Wild M3 10 60× magnification).
Embryos obtained from natural fertilization were selected at 24 hpf using a Discovery V8 stereomicroscope (Carl Zeiss, Germany), after which fish were raised at 28.5°C and maintained in dishes containing sterile E3 medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl, 0.33 mM MgSO4) in distilled water.
The human megakaryoblastic leukaemia cell line MEG-01 was purchased from ATCC (Rockville, MD), cultured in in RPMI-1640 with Glutamax (Invitrogen), 10% foetal bovine serum and 1% penicillin (100 IU/ml)/streptomycin (100 µg/ml), and maintained in plastic dishes in a humidified atmosphere at 37°C and 5% CO2.
Larvae were maintained in Petri dishes at a concentration of no more than 1 larvae/ml and collected on 5, 6 and 7 dpf for analysis by Oil Red O staining or their livers were dissected to collect RNA for gene expression analysis.
HeLa cells (human cervical cancer HeLa cell line was obtained from Cell Culture Center of Shanghai Science Academy, China) were maintained in Petri dishes at 37°C in a Dulbecco-modified Eagle culture medium supplemented with 10% fetal calf serum and 1% antibiotic mixture, under a humidified atmosphere containing 5% carbon dioxide.
The strains were maintained in Petri dishes on leaves of Pl. lanceolata in a growth chamber at 20 ± 2°C and a 16L/8D photoperiod, and they were transferred to fresh leaves approximately every 3 weeks.
