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Glycerol was intermittently added to the culture medium to maintain a concentration between 50 and 300 mM.
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Congenital iodide transport deficit (ITD) is an infrequent autosomic recessive condition characterized by inability of the thyroid gland to maintain a concentration gradient of iodide between the plasma and the thyroid follicular cell, resulting in hypothyroidism, diffuse or nodular goiter, and little or no uptake of radioiodine.
If this was the case, the supplementation rate was regulated to maintain a glycerol concentration between 50 and 300 mM during the cultivations.
THP-1 cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine and were maintained at a concentration between 2 and 10 × 105 cells per milliliter.
The steadily increased iodine concentration would maintain a certain concentration difference between solutions and specimen during the whole process.
For example, in a heterotrophic culture with C. cohnii, a non-specific nutritional limitation was detected, which coincided with a change from exponential to linear growth while continuously feeding glucose to maintain a residual concentration of between 5 and 20 g l−1 (de Swaaf et al. 2003c).
In the MIT group, continuous insulin infusion was started when the blood glucose concentrations exceeded 140 mg/dl in order to maintain a blood glucose concentration between 120 and 140 mg/dl.
In the CIT group, continuous insulin infusion was delivered when blood glucose concentrations exceeded 200 mg/dl; the insulin dose was then adjusted to maintain a blood glucose concentration between 180 and 200 mg/dl.
Second iteration (13C2 malonic acid to 13C3 malonic acid): The reaction volume was reduced back to 2.0 ml under vacuum to maintain a consistent reaction concentration between iterations.
Glucose (50%) was administered in order to maintain a blood glucose concentration between 3.5 and 6 mmol/l.
When the sodium nitrate concentration decreased to a level of 0.05 g/L, the feed stage was initiated; the nitrate feed was manually controlled to maintain a residual nitrate concentration between 0.10 and 0.6 g/L.
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