For a large number of laboratories worldwide studying proteomes in 'non-mainstream' organisms, annotations of tandem mass spectra data must rely on open reading frame (ORF) predictions from expressed sequence tag (EST) data from their species of interest or a phylogenetically close relative.
We suggest that interactions found in species such as T. pallidum be verified in more mainstream model organisms such as E. coli.
In this context, it is noteworthy that the Tat machinery is extensively used for protein secretion by certain halophilic archaea, such as Haloferax volcanii and Haloarcula hispanica, indicating that Tat has evolved into a mainstream secretion pathway for some organisms that grow in highly saline milieus [30], [31], [32], [33], [34], [35].
"I like being mainstream".
One word that is less understood by the masses, but is beginning to hit the agricultural mainstream is GMO (genetically modified organism).
Consequently, this technology is already providing important insights into the biological properties of various organisms, making it a mainstream component of biomedical research [ 3- 10].
DNA amplification (PCR) based tests are attractive due to their low cost and high speed and have become established as a mainstream diagnostic methodology for a variety of pathogenic organisms [ 29].
The mainstream media, blogosphere and just about every other living organism on Earth is still foaming at the mouth about Miley Cyrus's Video Music Award performance this past Sunday Night.
The intervening 70 years have, however, seen the emergence of organisms which are resistant to almost every antibiotic that has been introduced into mainstream use.
DNA microarray technology has been quickly adapted by mainstream laboratories to explore gene expression profiles of part or whole-genome for an organism [ 1, 2].
Thus, we present a fast and effective method to achieve gene-specific knockdown in adult organisms as well as developing Daphnia embryos that holds a tremendous potential to become a mainstream method in various types of biological studies that use Daphnia as a model organism.
