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Except for de Hoon et al. [ 10, 11, 21], which focused on B. subtilis, these methods were mainly validated using information from E. coli.
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This conclusion is mainly based on mechanistic studies performed on a widely used tumor cell line, but has also been validated using a cell model (human primary endothelial cells) relevant to nutrient-related pathologies like vascular ageing and diabetic complications.
The model was validated using field data.
The design was validated using SystemC.
Analytical results are validated using extensive simulations.
DCE analyses were validated using Stata 14.1.
The programs were validated using ANSYS.
Quantitative assays were validated using SYBR Green chemistry.
The results were validated using QPCR.
Structures were validated using Molprobity.
The results are validated using experimental datasets.
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