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Previous functional studies of SGs mainly performed using exogenous stressors or overexpressing/knocking down SG components.

The mineral characterization is mainly performed using micro-Raman Renishaw InVia Reflex apparatus.

Temperature monitoring after CA is mainly performed using a bladder probe as reported by 42%% of respondents, and/or an esophageal probe by 41%% of respondents (Additional file 2: Figure S3).

Based on the above analysis, at present, the numerical simulation of the CO2 displacement in porous media is mainly performed using the LBM, and few studies use conventional methods to simulate the displacement process.

Analyses of distribution of the phenotypic traits as well as QTL detection were mainly performed using the Qgene v. 4.3.0 program (Nelson 2005, http://www.qgene.org) and Windows QTL cartographer 2.5 (Wang et al. 2006b).

IEF is making a significant contribution to prefractionating proteins prior to conventional separation (2-DE and 2-DLC approaches), and to protein identification and characterization (mainly performed using MS).

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At present, gravity gradiometry surveys have mainly been performed using a helicopter.

Unfortunately, in the past, ROS measurements have mainly been performed using isolated mitochondria, a method which is prone to experimental artifacts and does not reflect the complexity of the in vivo process.

The available rare publications on low-velocity impact analysis of the functionally graded plates have mainly been performed using commercial finite element softwares and discrete models for variations of the material properties.

Genome-wide studies that address the impact of AS on proteome diversity have thus far mainly been performed using indirect evidence from transcriptomics data.

Recently, analysis of saliva for protein biomarker discovery has mainly been performed using two-dimensional difference gel electrophoresis (2D-DIGE) coupled with MS (which can identify around 300 proteins in a sample [ 59]), and liquid chromatograpy-MS (LC-MS) based techniques (which can identify more than 1,050 proteins in a sample; reviewed in [ 60]).

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