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For example, short disordered stretches are mainly negatively charged whereas long unstructured regions are either positively or negatively charged, but on average, nearly neutral [27].
Surface electrostatic potential maps confirm that the FMN domain of reductase is mainly negatively charged and the surface of the heme proximal side of aromatase is highly positively charged.
The increased current density of the –NH2 modified surface was attributed to a better orientation of the mainly negatively charged P. sordida CDH (isoelectric point = 5.7) on the electrode.
Specifically, the NLRP3 PYD is mainly negatively charged and thus was proposed to interact with the positively charged surface of the ASC PYD, while not competing with ASC2 for the negatively charged surface of the ASC PYD.
To ascertain whether Lap2β-EGFP-containing complexes were induced by Lap2β itself or by the EGFP tag on the overexpressed protein, we examined the overexpression of Lap2β fused to a short tag (FLAG) containing mainly negatively charged amino acids.
This observation allows to draw a conclusion that ASP adsorption decrease is related to developing the polymer chains due to the growth of the dissociation degree and particle charge changes; above the pH equal to 7.6 (pHpzc Cr2O3, [ 21]), there exist mainly negatively charged –CrO− groups on the chromium (III) oxide surface.
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These dots (S-CQDS), containing mainly a negatively charged surface due to dissociation of sulfonic/carboxylic groups and sulfates, showed a size dependent rather than a chemistry dependent (electrostatic interactions) inhibition of the Gram-positive bacterial growth.
As aggregation occurs, there is a significant fusogenic activity promoted by rBPI21, mainly on negatively charged liposomes (Figure 6).
The results demonstrate that all three proteins exhibit preferential, mainly irreversible adsorption onto negatively charged lipid bilayers, yet there was also significant variation in total uptake and the relative degree of adsorption onto negatively charged bilayers versus neutral and positively charged bilayers.
Thus, aggrecan interacting with hyaluronan, also highly negatively charged, mainly contributes to the polyanionic character of the PN.
The binding pocket surrounding this group is negatively charged (mainly due to D31 of HC CDR1 and D52 of HC CDR2; Fig. 2 D ), which is shown by calculation of surface electrostatics using the Fab structure without biocytinamide.
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