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As shown in Figure 4A, WT tetherin was mainly localized around the perinuclear region and the plasma membrane as dispersed dots.
Accounting for this, T3SS injected bacterial effector protein ExoS can be detected in 100% MEF cells under a similar infection condition, with injected ExoS mainly localized around paranuclear region (Fig. 1B).
Aquaporin-4 in the wild-type retina is mainly localized around blood vessels and at the ILM, whereas a weak staining was found in the inner and outer plexiform layers (Fig. 1E).
In addition to the main Mn K-edge, one can also study the 1s to 3d pre-edge spectra that arise from 1s to 3d transitions, by probing orbitals that are mainly localized around the metal ion.
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However, in cell, the proteasome complex is involved in degradation of proteins previously tagged with ubiquitin and is mainly localized in the nucleus and cytosol (around pH 7.5, [ 57]).
However, the inflammatory response of the endothelium remains mainly localized in and directly around viral patches within the tumor tissue.
In senescent cells, the fluorescence was mainly localized on cytoplasmic punctuated structures clustered around the nucleus, probably the damaged mitochondria (Figure 4B and C).
In contrast, 8 days following ligation, positive staining for ICAM-1 and P-selectin, were found in the gingivomucosal tissues from ligature operated rats, mainly localized in the inflammatory cells in derma and around the vessels respectively.
Immunostaining of fixed lung sections showed that Kv7.4-positive cells are mainly localized to blood vessels and form a ring around the blood vessel lumen (Fig. 5 A ). Figure 5 B shows the relative expression of KCNQ1, KCNQ4, and KCNQ5 mRNAs in pulmonary arteries from age-matched rats maintained for 3 days in a hypoxic or normoxic environment.
As previously shown [14], the dystrophin-associated protein, β-dystroglycan (β-DG) is mainly localized in Müller cells endfeet (Fig. 1F, open arrowhead), around blood vessels (Fig. 1F, filled arrow) and in the outer plexiform layer (Fig. 1F, filled arrowhead).
In control liver tissue, electron-dense gold particles showing the presence of D2-40 were few and mainly localized on the luminal site of lymphatic capillary endothelial cells around the portal tract.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com