To date, the selection of insect clones has been based mainly on laborious cell serial dilution methods which create few viable clones.
Despite the tremendous recent progress in this field, current efforts toward the directed evolution of these hybrid biocatalysts mainly rely on the laborious, individual purification of protein variants rendering the throughput, and hence the outcome of these campaigns feeble.
The treatise itself is mainly devoted to a laborious calculation of the various different possible forms of hypothetical syllogism involving two, three (with a first premise such as 'If it is A, then if it is B, it is C') and four terms (with a first premise such as 'If, if it is A then it is B, then, if it is C then it is D').
Although every data source takes into account different variables, aspects such as the human factor are not explicitly detailed, mainly because this factor is laborious to quantify.
This was mainly due to the rather laborious techniques, including Southern blotting, extensive cloning of DNA fragments and 'chromosome walking', needed to identify the affected gene.
Despite its potential interest, after this initial report, SPEX has not been widely applied mainly because of its intrinsic laborious protocol compared to other more innovative procedures (see "Next Generation Sequencing methodology" section).
These resources have facilitated the positional cloning of several agronomically important genes, but the functional validation of the candidate genes has relied mainly in transgenic approaches that are laborious, low throughput and require regulatory oversight.
The recent trend toward irregular shapes in high-rise building construction makes planning formwork layouts a more complex and laborious task that has been mainly conducted by a heuristic method based on the intuitive judgment of formwork engineers.
Setting the δ value in this fashion is laborious, making it unfeasible in most cases (mainly because of the need to know the entire collection).
Malaria parasite cloning is traditionally carried out mainly by using the limiting dilution method, which is laborious, imprecise, and unable to distinguish multiply-infected RBCs.
