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The cell cycle function (mainly cell cycle progression) was also enriched in GCs.
For example, U2AF1 mutations in myeloid neoplasms resulted in specific changes in splicing patterns affecting mainly cell cycle and RNA processing genes.
With the exception of SN-38 in A2780 cells, where the response was mainly cell cycle arrest even up to 100 ng ml−1, SN-38 drug concentrations used were those that resulted in approximately 10% apoptosis after a 3-day exposure.
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This process is PPI-linked mainly to processes related to protein catabolism, while it is expression-linked mainly to processes related to rRNA-processing, and GI-linked mainly to cell cycle related processes.
In summary, our findings demonstrate that CGZ and TGZ decrease cell proliferation mainly through cell cycle arrest.
The discordance observed when comparing the decrease in cell number assessed by MTT and apoptosis induction together with p21 induction strongly suggests that nutlin-3a mainly induced cell cycle arrest but not apoptosis in NHA.
The process "protein ubiquitination" (GO 0016567), for example, is predicted to be PPI-linked to processes related to protein catabolism while it is expression-linked to processes related to rRNA-processing and GI-linked mainly to cell cycle related processes.
The TFs in Cluster 7 mainly control cell cycle and root growth.
RITA-activated p53 predominantly affects apoptosis-related genes, whereas Nutlins mainly induce cell cycle regulators.
These protein-level features mainly reflect cell cycle regulation at the post-translational level, such as protein stability.
As shown in 11, the results highlight that processes corresponding mainly to cell cycle and metabolism were activated in MCF7 cells exposed to estrogen.
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