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Identification of ORFs and analysis of the BVMO4 gene and its deduced protein sequence was performed with CLCBio Main Workbench (Aarhus, Denmark) FGENESB (Soft Berry Mount Nysco, USA) and BLASTp at NCBI.
Different azo-reductase protein sequences from various microorganisms were aligned against those of B1 P. aeruginosa using the alignment tool of CLC Main Workbench 5 (CLC Bio, Aarhus, Denmark).
Chromatographs were visualised, inspected, assembled, aligned with related sequences and analysed using the CLC Main Workbench 5.5 (CLC bio, Denmark).
BLAST searches were performed in CLC Main Workbench (Version 6.0).
The sequences were analyzed using BioEdit Sequence Alignment Editor and CLC Main Workbench 6 (http://www.clcbio.com).
Amino acid alignments of CSEPs were carried out with the CLC main workbench (Aarhus, Denmark).
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The constructs were designed using the Gene Designer software and the CLC Main workbench.
Alignment to the chimpanzee, human (NC_012920) and mouse (NC_005089) reference sequences was accomplished using CLC Main Workbench 6.9 (Qiagen) and MUSCLE (Edgar, 2004).
The sequence data were analysed with CLC Main Workbench software (version 6.6.2, CLCbio, Aarhus, Denmark).
Results were screened using CLC Main Workbench sequence analysis software version 6.0.2 (CLC bio, Cambridge, MA).
Phylogenetic analyses were performed with CLC Main Workbench 6.6.2 (CLC bio, Aarhus Denmark) [ 80] and Mega5.
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