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All subsequent analysis was done only for main transcripts of each gene.
The modifications in expression of the main transcripts of the coagulation system were confirmed by qRT-PCR.
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As plasminogen is mainly synthesized in the kidney the results are in agreement with PLMN_HUMAN being the main transcript of the PLG gene and it seems that its expression is rapidly reduced in a deceased organism.
The altered cellular phenotype of fibroblasts and monocytes from CHM patients carrying knockouts and missense mutations of the CHM gene complement and expand the existing data resulting from knock down of the main transcript of the CHM gene in cultured human fetal RPE cells [47].
The main transcript of the MEN1 gene is a 2.8 kb mRNA.
This allowed us, solely based on the EST data, to predict the presence of two novel Jmjd6 splice variants in addition to the main transcript of 1.4 kb currently annotated in Ensembl.
To determine the effect of alternative transcription and splicing on mRNA stability, we compared decay rates identified with the best probe from the main transcript of each gene with decay rates estimated using other probes that matched the same gene.
To this end, we used the following two methods: (i) comparing mRNA decay rates in two independent experiments with different ES cell lines (MC1 and MC2-B6) cultured in the standard conditions and (ii) comparing mRNA decay rates estimated using different oligonucleotide probes on the microarray for the main transcript of the same gene.
There are three main transcripts producing three isoforms of UBE3A, and eight to ten additional transcripts of uncertain function.
These observations indicate that these genes are the main transcripts in developing xylem of Japanese cedar.
Whenever the sRNA transcript length could be predicted with reasonable certainty (Table 1), at least one of the main transcripts observed had the expected size.
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