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We have rewritten the main text explaining the model, and hope that this is now clear.
Additional comments have been added to the main text explaining that the bead motility assay does not included anionic lipids.
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Although it may be tempting to think of the ambiguity uncovered in (1) as an ambiguity in the verb 'believe', section 8 on Ambiguity theories in the main text explains why this is unattractive.
We clarify this aspect of our assay in the main text and explain in greater detail in the Methods section.
This is crucial part of the paper and the main text must explain carefully how this was done.
Indeed, the flux also contains a diffusive part, which effectively accounts for the actin production in the layer as we have now clarified in the main text, and explained in greater details in the appendices.
We have also added a statement to the main text and the Methods explaining how paired euploid controls were chosen: if known in advanced, the paired euploid was chosen as the most closely genetically related strain, based on phylogenetic analysis, but in two cases where we did not have a priori information we chose strains from the same ecological group.
It is not clear from the main text if the model explains the existence of medial nodes, how they are distributed, and how new ones form with cell length" 2) Please also address the following point from referee #3: "One minor improvement required for new figures (and some old figures) is that most Y-axes in the graphs should start from zero".
It is not clear from the main text if the model explains the existence of medial nodes, how they are distributed, and how new ones form with cell length" We have added 2 sentences to the paragraph 'Mathematical models for size-dependent accumulation of Cdr2p nodes' in the main paper to make it clear that the model does not specifically incorporate the details of nodal formation/growth.
If the second square also contains a free molecule (denoted by Y), complex formation can happen either between X's 5'-end and Y's 3'-end with a probability k 1 = 1 − exp (G X 5 ′ ~ 3 ′ Y ) or between Y's 5'-end 5'-ends 3'-end with androbability k ′ 1 = 1 − eXp (G Y 5 ′ ~ 3 ′ X ) (G is the binding energy between the two dangling-ends as explained in main text).
Abbreviations are explained in the main text body.
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