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We asked whether the main subpopulations of T and B lymphocytes or monocytes within mixed PBMC preparations were efficiently recovered and transfected for recombinant protein expression if treated with single HV or combined HV/LV pulses.

Here, by isolating single fibers from rat flexor digitorum brevis (FDB) muscle we were able to identify and clonally characterize two main subpopulations of SCs: the low proliferative clones (LPC) present in major proportion (∼75%) and the high proliferative clones (HPC), present instead in minor amount (∼25%).

There are two main subpopulations of T cells: CD4+ helper T and CD8+ cytotoxic T cells.

Among two main subpopulations of PB monocytes, CD14++CD16− and CD14+CD16++ [ 13], the latter possess an enhanced antitumour activity, as judged by an increased production of TNF, interleukin (IL -12, ROIL -12cytotoxic activity in vitROI[ 14].

To minimise the effect of a large variation in the number of cells plated due to the different size of these cell populations at extraction (figure 4B), we next performed single-cell sorting into 96-well plates, which showed that the three main subpopulations of cells have a very similar cloning efficiency in each experiment (figure 4D).

Similar(55)

Moreover, the LM38-HP subline presented a main subpopulation of small spindle cells, which could be an emergent third type of an otherwise insignificant subpopulation already present in LM38-LP cultures, or alternatively they could have evolved from the islet-forming epithelial cells, given that they present the same altered DNA ploidy.

M38 primary cultures and the LM38-LP cell line (passages 7 15) were composed of two main subpopulations: islets of small cobblestone-like epithelioid cells surrounded by loosely arranged large and clear spindle cells with large nuclei (Fig. 1, top panels).

As T lymphocytes are heterogeneous, an extensive analysis of their main subpopulations was made (Table 4).

This suggests the presence of 3 main subpopulations, hereinafter designated Q1, Q2 and Q3.

Profile regression revealed a typical grouping for the 829 subjects that consisted of three main subpopulations, with posterior means for the θ k parameters varying from θ3 = 0.09 in group 3 to θ1 = 0.15 in group 1.

When K was increased from three to five, each new cluster corresponded to one of the five main subpopulations - indica, aus, temperate japonica, tropical japonica and Group V. The tropical and temperate japonica groups diverged at K = 3, while the aus subpopulation emerged as an independent group at K = 4, and the Group V group emerged at K = 5.

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