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During the warm period, mean stream temperatures increased by 5.9 °C downstream along 9 km of the main stem from the headwater spring to the forested outflow as groundwater was exposed to air temperatures and environmental heat fluxes.
All axons originated from the cell body forming a main stem from which several thinner collaterals with many varicosities and terminal boutons arose.
Determinate habit (DH) was evaluated by scoring an increment of nodes in a main stem from R1 to maturity, according to Thseng and Hosokawa (1972).
Cytoplasmic cables extend along the growth axis of the main stem, from the base to the tip region where they form a fine, cortical F-actin cap mainly in a transverse pattern, highlighted by the brackets (C ).
PH was the average of four measurements per plot, and each measurement was recorded as the length of main stem from the ground to the top extremity of the plant at maturity.
Plant height was estimated by measuring the length of the main stem from the plant collar to the plant apex and the same plants were used to count the number of primary branches.
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Culm characters related lodging resistance, by using 15 representative main stems from each plot, was measured at 30 days after heading according to the method by Islam et al. (2007).
Digestibility of the main stems from transgenic (lines 4, 5) and WT plants.
The top and base 3 cm of main stems from 6-week-old plants (3 stems/line) were harvested and fixed overnight at 4°C in fixative solution (4% paraformaldehyde in 50 mM piperazine-N-N'-bis 2-ethanesulphonic acid), 5 mM EGTA, piperazine-N-N'-bis 2-ethanesulphonic
The first leaves from the two side shoots in the greenhouse experiments or, in the case of cuvette plants, one leaf from a side shoot, and the second, fifth and eighth leaves from the main stem (counted from the top) were sampled and frozen at –20 °C in order to measure soluble solids concentration (SSC) at a later date.
The three major upper tributaries of the Colorado – the Gunnison, Green, and San Juan – alone deliver almost per year to the main stem, mostly from snowmelt.
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